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superscript tm iii reverse transcriptase system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript tm iii reverse transcriptase system
    Superscript Tm Iii Reverse Transcriptase System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+tm+iii+reverse+transcriptase/superscript+iii+reverse+transcriptase/pm40609538-844-36-42
    Average 90 stars, based on 1 article reviews
    superscript tm iii reverse transcriptase system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Increased GHS-R1a expression in the hippocampus impairs memory encoding and contributes to AD-associated memory deficits
    Article Snippet: RNA quantity and quality were measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). .. Complementary DNA was synthesized from 1 μg of total RNA with SuperScript TM III Reverse Transcriptase (Invitrogen). .. PCR-based quantification of Ghsr1a was performed using a MasterCycler® ep realplex PCR system (Eppendorf) and a QuantiFast SYBR Green PCR Kit (Qiagen).

    Article Title: Tea leaf exosome-like nanoparticles (TELNs) improve oleic acid-induced lipid metabolism by regulating miRNAs in HepG-2 cells
    Article Snippet: .. The miRNA was synthesized by reverse transcription with SuperScript TM III reverse transcriptase (Thermo Fisher, USA). .. Quantitative PCR primers were designed with Primer Premier 6.0 and Beacon designer 7.8 software, and then synthesized by Shanghai Sangong Bioengineering Co.

    Article Title: Sirtuin1 (sirt1) regulates the glycolysis pathway and decreases cisplatin chemotherapeutic sensitivity to esophageal squamous cell carcinoma
    Article Snippet: .. Then Lipofectamine 3000 (Invitrogen, Carlsbad, CA) was used to transfect above plasmids (2 μg) into KYSE270 and EC-GI-10 cells for 48 h. QRT-PCR TRIzol reagent was used to isolate total RNA from the specimens and synthesized complementary DNA (cDNA) by a SuperScript TM III reverse transcriptase (Invitrogen). .. The RNA concentration and purity were measured using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    Reverse Transcription:

    Article Title: Increased GHS-R1a expression in the hippocampus impairs memory encoding and contributes to AD-associated memory deficits
    Article Snippet: RNA quantity and quality were measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). .. Complementary DNA was synthesized from 1 μg of total RNA with SuperScript TM III Reverse Transcriptase (Invitrogen). .. PCR-based quantification of Ghsr1a was performed using a MasterCycler® ep realplex PCR system (Eppendorf) and a QuantiFast SYBR Green PCR Kit (Qiagen).

    Article Title: Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8
    Article Snippet: iQ SYBR Green Supermix , Bio-Rad , 1708880. .. SuperScript TM III Reverse Transcriptase , ThermoFisher Scientific , 18080044. .. Cycloheximide , Cell Signaling Technology , 2112.

    Article Title: The Folding of Germ Granule mRNAs Controls Intermolecular Base Pairing in Germ Granules and Maintains Normal Fly Development
    Article Snippet: Total RNA was extracted using chloroform (Thermo Fisher: C298-500) and precipitated in isopropanol following the TRIzol Reagent User Guide. .. Next, about 2 μg of total RNA was treated with RQ1 RNase-Free DNase (Promega: M6101) following the manufacturer’s instructions. cDNA synthesis was performed using SuperScript TM III reverse transcriptase (Thermo Fisher: 18080093) following the manufacturer’s protocol. .. For each 10 μL of qRT-PCR reaction, approximately 100 ng of cDNA, 1.5 μL of 1 μM forward and reverse primers, and 5 μL iTaq universal SYBR® Green supermix (Bio-Rad: 1725122) were added.

    Article Title: A new cancer/testis long noncoding RNA, the OTP-AS1 RNA
    Article Snippet: .. To determine the 3’ end of the transcript reverse transcription (RT) followed by 2-round PCR were performed. cDNA from 293T cells RNA was obtained using SuperScript TM III Reverse Transcriptase (Invitrogen) with oligo(dT) adapter primer (5ʹ-GGCCAGGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3ʹ) according to manufacturer’s protocol. ..

    Article Title: The Multi-targeted Effect of Fascaplysin on the Proliferation and Dedifferentiation of Schwann Cells Inhibits Peripheral Nerve Degeneration by Blocking CDK4/6 and Androgen Receptor
    Article Snippet: Specific signals were visualized using enhanced chemiluminescence (ECL Select TM Western Blotting Detection Reagent, Cat#RPN2235, Amersham TM , Amersham, UK) as per the manufacturer’s instruction, and β-actin (#a5441, Sigma-Aldrich) was used as a loading control. .. Total RNA was isolated from SW10 cell lysates or sciatic nerve explants with or without Fas treatment using TRizol ® as per the manufacturer’s instructions (#15596-026, Invitrogen), and complementary DNA (cDNA) was reverse-transcribed using a SuperScript TM III reverse transcriptase (#18080044, Invitrogen). .. RT-qPCR was performed with a thermal cycler (TP700, Takara, Kusatsu, Japan) with 45 cycles, using 50 ng cDNA, 200 pmole/μL gene-specific primers (Supporting Table S1), and TB green premix Ex Taq (#RR420A, Takara).

    Article Title: The Helicobacter pylori orphan ATTAAT-specific methyltransferase M.Hpy99XIX plays a central role in the coordinated regulation of genes involved in iron metabolism
    Article Snippet: .. One μg of RNA was used for cDNA synthesis using the SuperScript TM III Reverse Transcriptase (Thermo Fischer Scientific, Darmstadt, Germany) as described previously [ ]. qPCR was performed with gene specific primers (Table S1) and SYBR Green Master Mix (Qiagen, Hilden, Germany). .. Reactions were run in a BioRad CFX96 system.

    Article Title: Tea leaf exosome-like nanoparticles (TELNs) improve oleic acid-induced lipid metabolism by regulating miRNAs in HepG-2 cells
    Article Snippet: .. The miRNA was synthesized by reverse transcription with SuperScript TM III reverse transcriptase (Thermo Fisher, USA). .. Quantitative PCR primers were designed with Primer Premier 6.0 and Beacon designer 7.8 software, and then synthesized by Shanghai Sangong Bioengineering Co.

    Article Title: Sirtuin1 (sirt1) regulates the glycolysis pathway and decreases cisplatin chemotherapeutic sensitivity to esophageal squamous cell carcinoma
    Article Snippet: .. Then Lipofectamine 3000 (Invitrogen, Carlsbad, CA) was used to transfect above plasmids (2 μg) into KYSE270 and EC-GI-10 cells for 48 h. QRT-PCR TRIzol reagent was used to isolate total RNA from the specimens and synthesized complementary DNA (cDNA) by a SuperScript TM III reverse transcriptase (Invitrogen). .. The RNA concentration and purity were measured using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

    cDNA Synthesis:

    Article Title: The Folding of Germ Granule mRNAs Controls Intermolecular Base Pairing in Germ Granules and Maintains Normal Fly Development
    Article Snippet: Total RNA was extracted using chloroform (Thermo Fisher: C298-500) and precipitated in isopropanol following the TRIzol Reagent User Guide. .. Next, about 2 μg of total RNA was treated with RQ1 RNase-Free DNase (Promega: M6101) following the manufacturer’s instructions. cDNA synthesis was performed using SuperScript TM III reverse transcriptase (Thermo Fisher: 18080093) following the manufacturer’s protocol. .. For each 10 μL of qRT-PCR reaction, approximately 100 ng of cDNA, 1.5 μL of 1 μM forward and reverse primers, and 5 μL iTaq universal SYBR® Green supermix (Bio-Rad: 1725122) were added.

    Article Title: The Helicobacter pylori orphan ATTAAT-specific methyltransferase M.Hpy99XIX plays a central role in the coordinated regulation of genes involved in iron metabolism
    Article Snippet: .. One μg of RNA was used for cDNA synthesis using the SuperScript TM III Reverse Transcriptase (Thermo Fischer Scientific, Darmstadt, Germany) as described previously [ ]. qPCR was performed with gene specific primers (Table S1) and SYBR Green Master Mix (Qiagen, Hilden, Germany). .. Reactions were run in a BioRad CFX96 system.

    Polymerase Chain Reaction:

    Article Title: A new cancer/testis long noncoding RNA, the OTP-AS1 RNA
    Article Snippet: .. To determine the 3’ end of the transcript reverse transcription (RT) followed by 2-round PCR were performed. cDNA from 293T cells RNA was obtained using SuperScript TM III Reverse Transcriptase (Invitrogen) with oligo(dT) adapter primer (5ʹ-GGCCAGGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3ʹ) according to manufacturer’s protocol. ..

    Isolation:

    Article Title: The Multi-targeted Effect of Fascaplysin on the Proliferation and Dedifferentiation of Schwann Cells Inhibits Peripheral Nerve Degeneration by Blocking CDK4/6 and Androgen Receptor
    Article Snippet: Specific signals were visualized using enhanced chemiluminescence (ECL Select TM Western Blotting Detection Reagent, Cat#RPN2235, Amersham TM , Amersham, UK) as per the manufacturer’s instruction, and β-actin (#a5441, Sigma-Aldrich) was used as a loading control. .. Total RNA was isolated from SW10 cell lysates or sciatic nerve explants with or without Fas treatment using TRizol ® as per the manufacturer’s instructions (#15596-026, Invitrogen), and complementary DNA (cDNA) was reverse-transcribed using a SuperScript TM III reverse transcriptase (#18080044, Invitrogen). .. RT-qPCR was performed with a thermal cycler (TP700, Takara, Kusatsu, Japan) with 45 cycles, using 50 ng cDNA, 200 pmole/μL gene-specific primers (Supporting Table S1), and TB green premix Ex Taq (#RR420A, Takara).

    Real-time Polymerase Chain Reaction:

    Article Title: The Helicobacter pylori orphan ATTAAT-specific methyltransferase M.Hpy99XIX plays a central role in the coordinated regulation of genes involved in iron metabolism
    Article Snippet: .. One μg of RNA was used for cDNA synthesis using the SuperScript TM III Reverse Transcriptase (Thermo Fischer Scientific, Darmstadt, Germany) as described previously [ ]. qPCR was performed with gene specific primers (Table S1) and SYBR Green Master Mix (Qiagen, Hilden, Germany). .. Reactions were run in a BioRad CFX96 system.

    SYBR Green Assay:

    Article Title: The Helicobacter pylori orphan ATTAAT-specific methyltransferase M.Hpy99XIX plays a central role in the coordinated regulation of genes involved in iron metabolism
    Article Snippet: .. One μg of RNA was used for cDNA synthesis using the SuperScript TM III Reverse Transcriptase (Thermo Fischer Scientific, Darmstadt, Germany) as described previously [ ]. qPCR was performed with gene specific primers (Table S1) and SYBR Green Master Mix (Qiagen, Hilden, Germany). .. Reactions were run in a BioRad CFX96 system.



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